A group of assassin fly pupae preserved in a single piece of Eocene amber

 

Authors: Haug JT, Nagler C, Haug C, Hörnig MK

Published in: Bulletin of Geosciences, volume 92, issue 3; pages: 283 - 295; Received 14 July 2016; Accepted in revised form 5 July 2017; Online 3 August 2017

Keywords: fossil pupa, Asilidae, Laphriinae, amber, fossilised behaviour,

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Abstract

Holometabolous insects represent a mega-diverse group of organisms that are dominant in most terrestrial faunas. All holometabolous insects develop via a specific transitory stage between the last larval stage and the adult, called the pupa. While insects in general have a comparably good fossil record, fossils of pupae of holometabolous insects are relatively rare. We report here four pupal specimens preserved in a single piece of amber. These represent pupa stages of assassin flies, Asilidae, and are most likely representatives of Laphriinae. While dipterans are quite common in the fossil record, especially in amber, representatives of Asilidae are comparably rare. Combining the rarity of the systematic group and the rarity of the specific life stage, these fossil remains of assassin fly pupae are extremely unusual; to date only a single specimen has been depicted in the literature. We discuss the importance of our new finding and possible interpretations regarding behavioural aspects of the group enclosed in amber.

References

ANDERSEN, T., BARANOV, V., GORAL, T., LANGTON, P., PERKOVSKY, E. & SYKES, D. 2015. First record of a Chironomidae pupa in amber. Geobios 48(4), 281-286.View article

BAKER, N.T. & FISHER, R.L. 1975. A taxonomic study of the Asilidae of Michigan. The Great Lakes Entomologist 8, 28-92.

BARRIGA, T.J.E. 1990. Parasites and larval predators of Cerambycidae and Buprestidae (Coleoptera) of Chile. Revista Chilena de Entomología 18, 57-60.

BRANDAO, C.R.F., URBANI, C.B., WAGENSBERG, J. & YAMAMOTO, C.I. 1998. New Technomyrmex in Dominican amber (Hymenoptera: Formicidae), with a reappraisal of Dolichoderinae phylogeny. Insect Systematics & Evolution 29(4), 411-428.View article

BRAUER, F. 1883. Die Zweiflügler des Kaiserlichen Museums zu Wien. III. Systematische Studien auf Grundlage der Dipteren-Larven nebst einer Zusammenstellung von Beispielen aus der Literatur über dieselben und Beschreibung neuer Formen. Denkschriften der Kaiserlichen Akademie der Wissenschaften. Mathematisch-Naturwissenschaftliche Classe 47, 1-100.

BRODEUR, J. & BOIVIN, G. 2004. Functional ecology of immature parasitoids. Annual Review of Entomology 49, 27-49.View article

BROMLEY, S.W. 1934, The laphriine robber flies of North America (Diptera: Asilidae). 359 pp. Ph.D. thesis, The Ohio State University, Columbus, USA.

CANNINGS, R.A. 1989. The robber flies (Diptera: Asilidae) of a Festuca Grassland in the Okanagan Valley, British Columbia. Journal of the Entomological Society of British Columbia 86, 14-26.

CANNINGS, R.A. 1997. Robber flies (Diptera: Asilidae) of the Yukon, 637-662. In DANKS, H.V. & DOWNES, J.A. (eds) Insects of the Yukon. Biological Survey of Canada (Terrestrial Arthropods), Ottawa.

CASTELO, M.K. & CAPURRO, A.F. 2000. Especificidad y densodependencia inversa en parasitoides con oviposicíon fuera del hospedador: el caso de Mallophora ruficauda (Diptera: Asilidae) en la pampa Argentina. Ecología Austral 10, 89-101.

CASTELO, M.K. & CORLEY, J.C. 2004. Oviposition behavior in the robber fly Mallophora ruficauda (Diptera: Asilidae). Annals of the Entomological Society of America 97, 1050-1054.View article

CASTELO, M.K. & CRESPO, J.E. 2012. Incidence of non-immunological defences of soil white grubs on parasitism success of Mallophora ruficauda larva (Diptera: Asilidae). Insects 3(3), 692-708.View article

CASTELO, M.K. & LAZZARI, C.R. 2004. Host-seeking behavior in larvae of the robber fly Mallophora ruficauda (Diptera: Asilidae). Journal of Insect Physiology 50(4), 331-336.View article

CASTELO, M.K., NEY-NIFLE, M., CORLEY, J.C. & BERNSTEIN, C. 2006. Oviposition height increases parasitism success by the robber fly Mallophora ruficauda (Diptera: Asilidae). Behavioral Ecology and Sociobiology 61(2), 231-243.View article

CEREZKE, H.F. 1973. Some parasites and predators of Hylobius warreni in Alberta. Bimonthly Research Notes 29, 24-25.

CRESPO, J.E. & CASTELO, M.K. 2009. Insights to host discrimination and host acceptance behaviour in a parasitoid (Diptera: Asilidae): Implications for fitness. Journal of Insect Physiology 55, 1072-1078.View article

DAVIS, S.R., ENGEL, M.S. & REN, D. 2010. A pupal caddisfly from the Early Cretaceous of China (Trichoptera). Cretaceous Research 31(4), 396-399.View article

DENNIS, D.S. 2012. Ethology of Proctacanthus brevipennis (Wiedemann, 1828) (Diptera: Asilidae) in Northeastern Florida, USA. Journal of the Entomological Research Society 14, 91-109.

DENNIS, D.S. 2013. Ethology of Stichopogon trifasciatus (Say, 1823) (Diptera: Asilidae) in Northeastern Florida, USA. Journal of the Entomological Research Society 15, 37-50.

DENNIS, D.S. & BARNES, J.K. 2012. The pupal case of a Palearctic robber fly, Andrenosoma atrum (Linnaeus) (Diptera: Asilidae). Proceedings of the Entomological Society of Washington 114, 16-22.View article

DENNIS, D.S. & BARNES, J.K. 2013. Pupal cases of four Nearctic species of Laphria (Diptera: Asilidae). Zootaxa 3681(4), 478-492.View article

DENNIS, D.S. & BARNES, J.K. 2014. The pupal case of Nearctic and Neotropical robber fly Andrenosoma cruentum (McAtee, 1919) (Diptera: Asilidae). Journal of the Entomological Research Society 16, 51-60.

DENNIS, D.S. & KNUTSON, L. 1988. Descriptions of pupae of South American robber flies (Diptera: Asilidae). Annals of the Entomological Society of America 81, 851-864.View article

DENNIS, D.S. & LAVIGNE, R.J. 1979. Ethology of Machimus callidus with incidental observations on M. occidentalis in Wyoming (Diptera: Asilidae). Pan-Pacific Entomologist 55, 208-221.

DENNIS, D.S., BARNES, J.K. & KNUTSON, L. 2013. Review and analysis of information on the biology and morphology of immature stages of robber flies (Diptera: Asilidae). Zootaxa 3673, 1-64.View article

DENNIS, D.S., BARNES, J.K. & KNUTSON, L. 2008. Pupal cases of Nearctic robber flies (Diptera: Asilidae). Zootaxa 1868, 1-98.View article

DENNIS, D.S., LAVIGNE, R.J. & BULLINGTON, S.W. 1986. Ethology of Efferia cressoni with a review of the comparative ethology of the genus (Diptera: Asilidae). Proceedings of the Entomological Society of Washington 88, 42-55.

DIKOW, T. 2009. A phylogenetic hypothesis for Asilidae based on a total evidence analysis of morphological and DNA sequence data (Insecta: Diptera: Brachycera: Asiloidea). Organisms Diversity & Evolution 9(3), 165-188.View article

DIKOW, T. & BAYLESS, K.M. 2009. Taxonomic revision of the genus Schildia Aldrich, 1923 (Diptera: Asilidae: Leptogastrinae) with the description of new extant and extinct species. Insect Systematics & Evolution 40(3), 253-289.View article

DIKOW, T. & GRIMALDI, D.A. 2014. Robber flies in Cretaceous ambers (Insecta: Diptera: Asilidae). American Museum Novitates 3799, 1-19.View article

DONLEY, D.E. 1978. Oviposition by the red oak borer, Enaphalodes rufulus (Coleoptera: Cerambycidae). Annals of the Entomological Society of America 71, 496-498.View article

DUFOUR, L. 1850. Recherches pour server a l’historie des métamorphoses des Asiliques. Annales des Science Naturalles, Series 3, Zoology 13, 141-158.

EVENHUIS, N.L. 1994. Catalogue of the fossil flies of the world (Insecta: Diptera). 608 pp. Backhuys Publishers, Leiden.

FIERKE, M.K., KINNEY, D.L., SALISBURY, V.B., CROOK, D.J. & STEPHEN, F.M. 2005. Development and comparison of intensive and extensive sampling methods and preliminary within-tree population estimates of red oak borer (Coleoptera: Cerambycidae) in the Ozark Mountains of Arkansas. Environmental Entomology 34, 184-192.View article

FISCHER, E.M. 1983. Pilica formidolosa (Mosca Asesina, Robber Fly), 755-758. In JANZEN, D.H. (ed.) Costa Rican Natural History. University of Chicago Press, Chicago.

FISCHER, T.C. 2015. Pupal exuvia of an adelid case-bearing moth (Lepidoptera) from Bitterfeld amber (Eocene). Zitteliana 55, 41-43.

GELLER-GRIMM, F. 1998. Protoloewinella keilbachi Schumann, 1984 from the Baltic amber (Diptera: Asilidae). Studia Dipterologica 5(1), 84.

GELLER-GRIMM, F. 2002. Robber flies (Diptera: Asilidae) of the Socotra Archipelago, Yemen. Fauna of Arabia 19, 467-489.

GODFRAY, H.C.J. 1994. Parasitoids: behavior and evolutionary ecology. 488 pp. Princeton University Press, Princeton.View article

GREENE, C. T. 1917. A contribution to the biology of North American Diptera. Proceedings of the Entomological Society of Washington 1, 146-161

GRIMALDI, D.A. 1990. Insects from the Santana Formation, Lower Cretaceous, of Brazil. Bulletin of the American Museum of Natural History 195, 1-191.

GRIMALDI, D.A., & CUMMING, J.M. 1999. Brachyceran Diptera in Cretaceous ambers and Mesozoic diversification of the Eremoneura. Bulletin of the American Museum of Natural History 239, 1-124.

GRIMALDI, D.A. & Engel, M.S. 2005. Evolution of the insects. 755 pp. Cambridge University Press, New York.

GRÖHN, C. 2015. Einschlüsse im Baltischen Bernstein. 424 pp. Wachholtz, Kiel.

HAUG, J.T., AUDO, D., CHARBONNIER, S. & HAUG, C. 2013a. Diversity of developmental patterns in achelate lobsters-today and in the Mesozoic. Development Genes & Evolution 223, 363-373.View article

HAUG, J.T., HÄDICKE, C.W., HAUG, C. & HÖRNIG, M.K. 2015b. A possible hatchling of a jumping bristletail in 50 million years old amber. Neues Jahrbuch für Geologie und Paläontologie, Abhandlungen 278, 191-199.View article

HAUG, J.T., HAUG, C. & GARWOOD, R. 2016. Evolution of insect wings and development - new details from Palaeozoic nymphs. Biological Reviews 91, 53-69.View article

HAUG, J.T., MARTIN, J.W. & HAUG, C. 2015a. A 150-million-year-old crab larva and its implications for the early rise of brachyuran crabs. Nature Communications 6, art. 6417.View article

HAUG, J.T., MÜLLER, C.H.G. & SOMBKE, A. 2013b. A centipede nymph in Baltic amber and a new approach to document amber fossils. Organisms Diversity & Evolution 13, 425-432.View article

HAYAT, R. 1997. Prey of some robber flies (Diptera: Asilidae) in Turkey. Zoology in the Middle East 15, 87-94.View article

HÖRNIG, M.K., SOMBKE, A., HAUG, C., HARZSCH, S. & HAUG, J.T. 2016. What nymphal morphology can tell us about parental investment - a group of cockroach hatchlings in Baltic Amber documented by a multi-method approach. Palaeontologia Electronica 19(1), art. 5A.View article

HUGUENEY, M., TACHET, H., & ESCUILLIÉ, F. 1990. Caddisfly pupae from the Miocene indusial limestone of SaintGérand-le-Puy, France. Palaeontology 33(2), 495-502.

HULL, F.M. 1962. Robber flies of the world. Bulletin of the United States National Museum 224(1-2), 1-907.View article

JOERN, A. & RUDD, N.T. 1982. Impact of predation by the robber fly Proctacanthus milbertii (Diptera: Asilidae) on grasshopper (Orthoptera: Acrididae) populations. Oecologia 52, 42-46.View article

JOHNSTON, J.E. & BORKENT, A. 1998. Chaoborus lichtenstein (Diptera: Chaoboridae) pupae from the middle Eocene of Mississippi. Journal of Paleontology 72(3), 491-493.View article

KNUTSON, L. 1972. Pupa of Neomochtherus angustipennis (Hine), with notes on feeding habits of robber flies and a review of publications on morphology of immature stages (Diptera: Asilidae). Proceedings of the Biological Society of Washington 85, 163-178.

LAVIGNE, R.J. & DENNIS, D.S. 1975. Ethology of Efferia frewingi (Diptera: Asilidae). Annals of the Entomological Society of America 68, 992-996.View article

LAVIGNE, R.J. & DENNIS, D.S. 1980. Ethology of Proctacanthella leucopogon in Mexico (Diptera: Asilidae). Proceedings of the Entomological Society of Washington 82, 260-268.

LAVIGNE, R.J., DENNIS, D.S. & GOWEN, J.A. 1978. Asilid literature update 1956-1976. University of Wyoming Agriculture Experiment Station Science Monograph 36, 1-134.

LEHR, P.A. 1977. Robber flies from the subfamilies Atomosiinae and Laphriinae (Diptera, Asilidae) from Central Asia and Kazakhstan. Entomological Review 56, 123-131.

LUKASHEVICH, E. 2012. Pupae of Mesozoic Oryctochlus Kalugina, 1985 (Chironomidae: Podonominae), with description of two new species. Fauna Norvegica 31, 1-159.View article

LUKASHEVICH, E. & PRZHIBORO, A.A. 2015. A new tribe of Diamesinae (Diptera: Chironomidae) from the Lower Cretaceous of Mongolia. Cretaceous Research 52, 562-569.View article

MELIN, D.E. 1923. Contributions to the knowledge of the biology, metamorphosis and distribution of the Swedish asilids in relation to the whole family of asilids. Zoologiska Bidrag Fran Uppsala 8, 1-137.

MINELLI, A., BRENA, C., DEFLORIAN, G., MARUZZO, D. & FUSCO, G. 2006. From embryo to adult-beyond the conventional periodization of arthropod development. Development Genes & Evolution 216(7-8), 373-383.View article

MUSSO, J.J. 1967 Étude de la nymphe d’Andrenosoma bayardi Séguy, 1952 (Dipt. Asilidae). Bulletin de la Société entomologique de France 72, 75-80.View article

MUSSO, J.J. 1978 Recherches sur le développement, la nutrition et 1’ecologia des Asilidae (Diptera-Brachycera). 312 pp. Ph.D. thesis, Universite de Droit d’Economie et des Sciences d’Aix-Marseille, Marseille, France.

MUSSO, J.J. 1981. Morphology and development of the immature stages of some robber flies (Diptera: Brachycera: Asilidae). Entomologia Generalis 7, 89-104.View article

MUSSO, J.J. 1983. Nutritive and ecological requirements of robber flies (Diptera: Brachycera: Asilidae). Entomologia Generalis 9, 35-50.View article

OLDROYD, H. 1972. Two robber flies (Diptera: Asilidae) of unusual structure. Journal of Natural History 6, 635-642.View article

PAPE, T., BLAGODEROV, V. & MOSTOVSKI, M.B. 2011. Order Diptera Linnaeus 1785. Zootaxa 3148, 222-229.View article

PERKOVSKY, E.E. 2008. First occurrence of syninclusion of ants Lasius schiefferdeckeri Mayr (Hymenoptera, Formicidae) and aphids Germaraphis ungulata Heie (Homoptera, Aphidinea) in amber (Klesov). Russian Entomological Journal 17(2), 207-208.

POINAR, G. 2004. Evidence of parasitism by Strepsiptera in Dominican amber. Biocontrol 49(3), 239-244.View article

PRITCHARD, A.E. 1935. New Asilidae from the Southwestern United States (Diptera). American Museum Novitates 813, 1-14.

RITCHER, P.O. 1940. Kentucky white grubs. Kentucky Agricultural Experiment Station Bulletin 401, 71-157. RUDOLF, N.R., HAUG, C. & HAUG, J.T. 2016. Functional morphology of giant mole crab larvae: a possible case of defensive enrollment. Zoological Letters 2, art. 17.

SCARBROUGH, A.G., & POINAR, JR., G.O. 1992. Upper Eocene robber flies of the genus Ommatius (Diptera: Asilidae) in Dominican amber. Insecta Mundi 6(1), 13-18.

SCHUMANN, H. 1984. Erstnachweis einer Raubfliege aus dem Sächsischen Bernstein (Diptera: Asilidae). Deutsche Entomologische Zeitschrift 31, 217-223.

SHUROVNEKOV, B.G. 1962. Field entomophagous predators (Coleoptera, Carabidae, and Diptera, Asilidae) and factors determining their efficiency. Entomological Review 41, 476-485.

STUBBS, A.E. & DRAKE, M. 2001. British soldierflies and their allies. An illustrated guide to their identification and ecology, covering all flies (Diptera) in the families Acroceridae, Asilidae, Athericidae, Bombyliidae, Rhagionidae, Scenopinidae, Stratiomyidae, Tabanidae, Therevidae, Xylomyidae and Xylophagidae. 528 pp. British Entomological and Natural History Society, Reading.

VELTZ, I., AZAR, D. & NEL, A. 2007. New chironomid flies in Early Cretaceous Lebanese amber (Diptera: Chironomidae). African Invertebrates 48(1), 169-191.

WEDMANN, S., HOERNSCHEMEYER, T. & SCHMIED, H. 2011. Fossil water-penny beetles (Coleoptera: Psephenidae: Eubrianacinae) from the Eocene of Europe, with remarks on their phylogenetic position and biogeography. Palaeontology 54, 965-980.View article

WEISS, H.B. & WEST, E. 1922. The insects and plants of a moist woods on the Piedmont Plain of New Jersey. Journal of the New York Entomological Society 30(4), 169-191.

WEITSCHAT, W. & WICHARD, W. 1998. Atlas der Pflanzen und Tiere im Baltischen Bernstein. 256 pp. Dr. Friedrich Pfeil Verlag, München.

WHITE, A. 1916. The Diptera-Brachycera of Tasmania: Part III. Families Asilidae, Bombylidae, Empidae, Dolichopodidae and Phoridae. Papers & Proceedings of the Royal Society of Tasmania 1916, 148-266.View article

WOLFF, M. & LAMAS, C.J.E. 2016. Family Asilidae. Zootaxa 4122(1), 353-371.View article

WOOD, G.C. 1981. Asilidae. Manual of Nearctic Diptera 1, 549-573.